- Jun 6 Andrea Cesalpino, Italian philosopher, physician and botanist, born in Arezzo, Italy (d. 1603)
- Jun 12 Cosimo I de Medici, Grand Duke of Tuscany and art patron of Florence, born in Florence, Italy (d. 1574)
- Jun 24 Theodore Beza, French Reformed Protestant theologian, born in Vezelay, Burgundy, Kingdom of France (d. 1605)