- Apr 6 Joachim Vadianus, Swiss physician/mayor of Sankt Gallen, dies at 66
- Apr 21 Oda Nobuhide, Japanese warlord (b. 1510)
- May 18 Domenico di Pace Beccafumi, Italian Mannerist painter, dies at 65
- May 31 Aristotele "Basiano" da Sangallo, Italian set designer, dies
- Jul 13 John Wallop, English soldier and diplomat (b. 1490)
- Aug 26 Margareta Leijonhufvud, queen of Gustav I of Sweden (b. 1516)
- Sep 30 Ōuchi Yoshitaka, Japanese warlord (b. 1507)