- Sep 11 John de White, calvinist/banker to Prague, commits suicide
- Sep 17 Thomas Lake, English statesman (b. 1567)
- Sep 18 Melchior Klesl, Austrian cardinal and statesman (b. 1552)
- Sep 20 Claudio Saracini, Italian composer, dies at 44
- Sep 25 Ambrogio Spinola, Spanish marquis of Balbases, dies at about 61
- Sep 30 John Billington, English-born murderer who sailed on the Mayflower, 1st American executed by Plymouth colony, hanged at about 40